HIC1 Polyclonal antibody

HIC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24949-1-AP

Synonyms

hic 1, HIC1, hypermethylated in cancer 1, ZBTB29

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Validation Data Gallery

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  WB analysis of Jurkat using 24949-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24949-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 24949-1-AP is HIC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIC1 fusion protein Ag21849
• Full Name: hypermethylated in cancer 1
• Calculated Molecular Weight: 714 aa, 75 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC157027
• Gene Symbol: HIC1
• Gene ID (NCBI): 3090
• RRID: AB_2879816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.; Authors - Huan Wang
human	WB,IHC	Cancer Cell Int; ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression.; Authors - Junqiang Liu
human	IHC	Front Immunol; Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity; Authors - Yanlin Wu