HEXA Polyclonal antibody

HEXA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29259-1-AP

Synonyms

HEXA, Hexosaminidase subunit A, TSD

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Validation Data Gallery

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  WB analysis using 29259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29259-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 29259-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29259-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29259-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells
• Positive IHC detected in: human kidney tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEXA fusion protein Ag30808
• Full Name: hexosaminidase A (alpha polypeptide)
• Calculated Molecular Weight: 529 aa, 60 kDa
• Observed Molecular Weight: 50-54 kDa, 60-67 kDa
• GenBank Accession Number: BC018927
• Gene Symbol: HEXA
• Gene ID (NCBI): 3073
• RRID: AB_2918259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Publications

Species	Application	Title
human	WB	J Proteome Res; Proteome and Glycoproteome Analyses Reveal Regulation of Protein Glycosylation Site-Specific Occupancy and Lysosomal Hydrolase Maturation by N-Glycan-Dependent ER-Quality Control; Authors - Jingru Chen