HERPUD2 Monoclonal antibody

HERPUD2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10H1

Cat no : 68427-1-Ig

Synonyms

FLJ22313, FLJ31032, HERPUD family member 2, HERPUD2

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Validation Data Gallery

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  WB analysis using 68427-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68427-1-Ig (HERPUD2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HepG2 using 68427-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HERPUD2 antibody (68427-1-Ig, Clone: 1H10H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, L02 clls, HepG2 cells, A549 cells, HT-29 cells, LNCaP cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68427-1-Ig targets HERPUD2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: HERPUD2 fusion protein Ag15007
• Full Name: HERPUD family member 2
• Calculated Molecular Weight: 406 aa, 45 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: BC005091
• Gene Symbol: HERPUD2
• Gene ID (NCBI): 64224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).