HER2/ErbB2 Monoclonal antibody, PBS Only

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  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

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  FC experiment of SK-BR-3 using 60311-1-Ig (same clone as 60311-1-PBS)

  1x10^6 SK-BR-3 cells were surface stained with 0.2 μg HER2/ErbB2 Monoclonal antibody (60311-1-Ig, Clone: 1B12A7) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

HER2/ErbB2 Monoclonal Antibody for IHC, IF-P, ELISA

Cat No. 60311-1-PBS

Clone No.1B12A7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, ELISA

Synonyms

ERBB2, HER2, HER 2/neu, HER 2, CD340

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Product Information

60311-1-PBS targets HER2/ErbB2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HER2/ErbB2 fusion protein Ag16463
• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 1255 aa, 138 kDa
• GenBank Accession Number: BC156755
• Gene Symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• RRID: AB_2881423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04626
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HER2, ErbB2, and Neu is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.