HEMGN Polyclonal antibody

HEMGN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17616-1-AP

Synonyms

EDAG, EDAG 1, HEMGN, hemogen, Hemopoietic gene protein, NDR, PRO1620

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Validation Data Gallery

• 

  WB analysis of Jurkat using 17616-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17616-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17616-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17616-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17616-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 17616-1-AP

  IP result of anti-HEMGN (IP:17616-1-AP, 4ug; Detection:17616-1-AP 1:500) with HL-60 cells lysate 3000ug.

• 

  IHC staining of human testis using 17616-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17616-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells, human testis tissue, mouse testis tissue
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17616-1-AP targets HEMGN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEMGN fusion protein Ag11793
• Full Name: hemogen
• Calculated Molecular Weight: 483 aa, 55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC048324
• Gene Symbol: HEMGN
• Gene ID (NCBI): 55363
• RRID: AB_2117676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Biomedicines; Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response; Authors - Yunshu Zhu