HELIOS Monoclonal antibody, PBS Only

HELIOS Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F1F10

Cat no : 68558-1-PBS

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

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Validation Data Gallery

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  WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MOLT-4 using 68558-1-Ig (same clone as 68558-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

  IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

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Product Information

68558-1-PBS targets HELIOS in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HELIOS fusion protein Ag4357
• Full Name: IKAROS family zinc finger 2 (Helios)
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC028936
• Gene Symbol: HELIOS
• Gene ID (NCBI): 22807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).