HECW2 Polyclonal antibody

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  WB analysis using 32026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 32026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 32026-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 32026-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

HECW2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 32026-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NEDD4-like E3 ubiquitin-protein ligase 2, KIAA1301, HECT-type E3 ubiquitin transferase HECW2, HECT, C2 and WW domain-containing protein 2, EC:2.3.2.26

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Tested Applications

• Positive WB detected in: MDA-MB-468 cells, U-118 MG cells
• Positive IHC detected in: mouse cerebellum tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32026-1-AP targets HECW2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HECW2 fusion protein Ag36316
• Full Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
• Calculated Molecular Weight: 176 kDa，1572aa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: XM_017004563.1
• Gene Symbol: HECW2
• Gene ID (NCBI): 57520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P2P5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).