HEBP2 Polyclonal antibody

HEBP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12706-1-AP

Synonyms

C6orf34, C6ORF34B, HEBP2, heme binding protein 2, KIAA1244, Placental protein 23, PP23, Protein SOUL, SOUL



Tested Applications

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12706-1-AP targets HEBP2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEBP2 fusion protein Ag3387
Full Name heme binding protein 2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC008205
Gene Symbol HEBP2
Gene ID (NCBI) 23593
RRIDAB_2232981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEBP2(Heme-binding protein 2), also named SOUL and C6orf34, can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions (PMID: 17098234). It is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. hEBP2 is essential for the proliferation of human cells and the repression of hEBP2 severely decreases the ability of EBNA1 and EBV-based plasmids to bind mitotic chromosomes (PMID: 15923612).

Protocols

Product Specific Protocols
WB protocol for HEBP2 antibody 12706-1-APDownload protocol
IP protocol for HEBP2 antibody 12706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Oncol

High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance

Authors - BiKang Yang