HEBP1 Recombinant antibody

• Featured Product
• KD/KO Validated

HEBP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230156B6

Cat no : 82950-1-RR

Synonyms

Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP

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Validation Data Gallery

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  WB analysis using 82950-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82950-1-RR (HEBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 82950-1-RR

  WB result of HEBP1 antibody (82950-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

• 

  IHC staining of mouse liver using 82950-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 82950-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of U2OS using 82950-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug HEBP1 Recombinant antibody (82950-1-RR, Clone:230156B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82950-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82950-1-RR against Human HEBP1 were performed. The affinity constant is 74 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, L02 cells, HepG2 cells, rat liver tissue. mouse liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82950-1-RR targets HEBP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HEBP1 fusion protein Ag10538
• Full Name: heme binding protein 1
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC016277
• Gene Symbol: HEBP1
• Gene ID (NCBI): 50865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.