HEATR2 Polyclonal antibody

HEATR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24578-1-AP

Synonyms

HEAT repeat-containing protein 2, HEAT repeat containing 2, Dynein axonemal assembly factor 5, DNAAF5

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Validation Data Gallery

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  WB analysis using 24578-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24578-1-AP (HEATR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 24578-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24578-1-AP (HEATR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 24578-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24578-1-AP (HEATR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

24578-1-AP targets HEATR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEATR2 fusion protein Ag20080
• Full Name: HEAT repeat containing 2
• Calculated Molecular Weight: 855 aa, 94 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC047240
• Gene Symbol: HEATR2
• Gene ID (NCBI): 54919
• RRID: AB_2827668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEATR2 also termed as HEAT repeat containing 2 is an 855 amino acid protein. HEATR2 is a member of a family of ten other uncharacterized HEAT-repeat-containing proteins, which contains HEAT (Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A) and the yeast PI3-kinase Tor1) repeats. HEAT repeats form rod-like helical structures that are involved in intracellular transport. HEATR2 play an important role in dynein arm transportation or assembly. HEATR2 mutation is implicated in Primary ciliary dyskinesia 18.

Publications

Species	Application	Title
human	WB	Mol Cell; DNA Methylation Regulates Alternative Polyadenylation via CTCF and the Cohesin Complex.; Authors - Vishal Nanavaty
rat,human	WB, IF	PLoS Genet; HEATR2 Plays a Conserved Role in Assembly of the Ciliary Motile Apparatus.; Authors - Christine P Diggle
human	WB	Front Oncol; DNAAF5 promotes hepatocellular carcinoma malignant progression by recruiting USP39 to improve PFKL protein stability; Authors - Yaping Liu