HDGF Polyclonal antibody

• Featured Product
• KD/KO Validated

HDGF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 11344-1-AP

Synonyms

HMG-1L2, High mobility group protein 1-like 2, Hepatoma-derived growth factor

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Validation Data Gallery

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  WB analysis using 11344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11344-1-AP (HDGF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11344-1-AP

  IP result of anti-HDGF (IP:11344-1-AP, 3ug; Detection:11344-1-AP 1:1000) with HepG2 cells lysate 3000ug.

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 11344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HDGF antibody (11344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293T cells, Hela cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue, human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

11344-1-AP targets HDGF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDGF fusion protein Ag1893
• Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC018991
• Gene Symbol: HDGF
• Gene ID (NCBI): 3068
• RRID: AB_2232950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.

Publications

Species	Application	Title
human	WB	J Hazard Mater; Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.; Authors - Ruina Cui
human	IHC,WB	Biomaterials; Gint4.T-siHDGF chimera-capped mesoporous silica nanoparticles encapsulating temozolomide for synergistic glioblastoma therapy; Authors - Huaijun Fei
human	WB,IHC,IF,ChIP	Mol Oncol; Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.; Authors - Xuejie Min ; KD Validated
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang
human	WB	Eur J Cancer; Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.; Authors - Li Yan Li YL
human	WB	Hum Cell; LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer.; Authors - Qiuhong Ma ; KD Validated