HDAC9 Polyclonal antibody

HDAC9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28334-1-AP

Synonyms

DKFZp779K1053, HD7, HD7b, HD9, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR

---

Validation Data Gallery

• 

  WB analysis using 28334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28334-1-AP (HDAC9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 28334-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC9 antibody (28334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: BxPC-3 cells, MDA-MB-231 cells, MDA-MB-468 cells, mouse brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28334-1-AP targets HDAC9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC9 fusion protein Ag28514
• Full Name: histone deacetylase 9
• Calculated Molecular Weight: 1011 aa, 111 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC152405
• Gene Symbol: HDAC9
• Gene ID (NCBI): 9734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle, and testis. HDAC9 has 11 isoforms produced by alternative splicing. The observed molecular weight of HDAC9 is 57 kDa, which represents alternate isoforms of HDAC9.