HDAC6-specific Polyclonal antibody

HDAC6-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16167-1-AP

Synonyms

HDAC6, KIAA0901, Histone deacetylase 6, HDAC 6, EC:3.5.1.98

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Validation Data Gallery

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  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells, HT-1080 cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16167-1-AP targets HDAC6-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 6
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC005872
• Gene Symbol: HDAC6
• Gene ID (NCBI): 10013
• RRID: AB_10598012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. This antibody is specific to HDAC6. It does not cross react with other HDACs.

Publications

Species	Application	Title
mouse	WB,IHC	Oncogene; MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.; Authors - Jiajie Tu
rat	WB	J Cell Mol Med; HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.; Authors - He Min M
mouse	WB	Adv Sci (Weinh); Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis; Authors - Kun-Huan Zhang