Validation Data Gallery
|Positive WB detected in||K-562 cells, HepG2 cells, human liver tissue, HeLa cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human testis tissue, human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12834-1-AP targets HDAC6 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC6 fusion protein Ag3572|
|Full Name||histone deacetylase 6|
|Calculated molecular weight||1063 aa, 114 kDa, 131 kDa|
|Observed molecular weight||160 kDa|
|GenBank accession number||BC013737|
|Gene ID (NCBI)||10013|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.
|Product Specific Protocols|
|WB protocol for HDAC6 antibody 12834-1-AP||Download protocol|
|IHC protocol for HDAC6 antibody 12834-1-AP||Download protocol|
|IF protocol for HDAC6 antibody 12834-1-AP||Download protocol|
|IP protocol for HDAC6 antibody 12834-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Thorac Cardiovasc Surg
Histone deacetylase 6 reduction promotes aortic valve calcification via an endoplasmic reticulum stress-mediated osteogenic pathway.
Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.
Stabilized peptide HDAC inhibitors derived from HDAC1 substrate H3K56 for the treatment of cancer stem-like cells in vivo.
Histone deacetylases and acetylated histone H3 are involved in the process of hepatitis B virus DNA replication.
Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.
Mol Hum Reprod
RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.
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K (Verified Customer) (08-03-2021)
total cell lysate (20 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with this in blocking buffer (1:1000) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:1000) membranes were imaged with ECL and expected band was detected.