HDAC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

HDAC4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 17449-1-AP

Synonyms

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

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Validation Data Gallery

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  WB analysis using 17449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17449-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17449-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17449-1-AP

  IP Result of anti-HDAC4 (IP:17449-1-AP, 4ug; Detection:17449-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

17449-1-AP targets HDAC4 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC4 fusion protein Ag11572
• Full Name: histone deacetylase 4
• Calculated molecular weight: 972 aa, 106 kDa
• Observed molecular weight: 119-140 kDa
• GenBank accession number: BC039904
• Gene symbol: HDAC4
• Gene ID (NCBI): 9759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Publications

Species	Application	Title
mouse	IF	Nat Commun; TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling.; Authors - Jianwei Li
human	WB	Diabetes Metab Syndr Obes; Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.; Authors - Hang Zhao ; KD Validated
		Ann Clin Lab Sci; Expression of HDAC4 in Stage B Hepatocellular Carcinoma and Its Influence on Survival.; Authors - Zhonghong Wang
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated
human	WB	Front Genet; Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.; Authors - Zezhong Mou
mouse	WB,chIP	Biochim Biophys Acta; Exercise-induced GLUT4 transcription via inactivation of HDAC4/5 in mouse skeletal muscle in an AMPKα2-dependent manner.; Authors - Yanmei Niu