HDAC4 Polyclonal antibody

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  WB analysis using 17449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17449-1-AP

  IP result of anti-HDAC4 (IP:17449-1-AP, 4ug; Detection:17449-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of mouse liver using 17449-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (17449-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HDAC4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 17449-1-AP

Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, CoIP, chIP, ELISA

Synonyms

KIAA0288, HD4, EC:3.5.1.98

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293T cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

This antibody is not recommended for WB assays of mouse samples.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

17449-1-AP targets HDAC4 in WB, IHC, IF-P, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC4 fusion protein Ag11572
• Full Name: histone deacetylase 4
• Calculated Molecular Weight: 972 aa, 106 kDa
• Observed Molecular Weight: 119-140 kDa
• GenBank Accession Number: BC039904
• Gene Symbol: HDAC4
• Gene ID (NCBI): 9759
• RRID: AB_2118864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Publications

Species	Application	Title
	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
human	WB, IF	Hepatology; Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1.; Authors - Lucía Barbier-Torres ; KD Validated
	WB,IHC,IF	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang ; KD Validated
human	ChIP,CoIP	Mol Ther Nucleic Acids; Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity.; Authors - Qianqian Guo
human	WB	J Virol; IFI16 recruits HDAC1 and HDAC2 to deacetylate the Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA), facilitating latency; Authors - Anandita Ghosh
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated