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- KD/KO Validated
HDAC4 Polyclonal antibody
HDAC4 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 17449-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 6 publications below |
WB | See 18 publications below |
IHC | See 3 publications below |
IF | See 6 publications below |
CoIP | See 1 publications below |
ChIP | See 2 publications below |
Product Information
17449-1-AP targets HDAC4 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HDAC4 fusion protein Ag11572 |
Full Name | histone deacetylase 4 |
Calculated molecular weight | 972 aa, 106 kDa |
Observed molecular weight | 119-140 kDa |
GenBank accession number | BC039904 |
Gene symbol | HDAC4 |
Gene ID (NCBI) | 9759 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for HDAC4 antibody 17449-1-AP | Download protocol |
IHC protocol for HDAC4 antibody 17449-1-AP | Download protocol |
IP protocol for HDAC4 antibody 17449-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Diabetes Metab Syndr Obes Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.
| ||
Ann Clin Lab Sci Expression of HDAC4 in Stage B Hepatocellular Carcinoma and Its Influence on Survival. | ||
J Cell Mol Med Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.
| ||
Front Genet Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. | ||
Biochim Biophys Acta Exercise-induced GLUT4 transcription via inactivation of HDAC4/5 in mouse skeletal muscle in an AMPKα2-dependent manner. |