HDAC3-specific Polyclonal antibody

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• KD/KO Validated

HDAC3-specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16164-1-AP

Synonyms

HD3, HDAC3, HDAC3-specific, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Validation Data Gallery

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  WB analysis of HeLa using 16164-1-AP

  WB result of HDAC3-specific antibody (16164-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3-specific transfected HeLa cells.

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  WB analysis using 16164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 16164-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, human stomach tissue, HeLa cells, HL-60 cells, MOLT-4 cells, NCI-H1299 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16164-1-AP targets HDAC3-specific in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 3
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_003883
• Gene Symbol: HDAC3
• Gene ID (NCBI): 8841
• RRID: AB_2118384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.