HDAC3 Recombinant antibody, PBS Only

HDAC3 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2N1

Cat no : 81211-1-PBS

Synonyms

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Validation Data Gallery

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  WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 81211-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81211-1-RR against Human HDAC3 were performed. The affinity constant is 0.213 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81211-1-PBS targets HDAC3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HDAC3 fusion protein Ag0396
• Full Name: histone deacetylase 3
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC000614
• Gene Symbol: HDAC3
• Gene ID (NCBI): 8841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.