HDAC10 Recombinant antibody

HDAC10 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240877E1

Cat no : 83788-4-RR

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

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Validation Data Gallery

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  WB analysis of Jurkat using 83788-4-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 83788-4-RR (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83788-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83788-4-RR against Human HDAC10 were performed. The affinity constant is 0.476 nM.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83788-4-RR is HDAC10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HDAC10 fusion protein Ag18851
• Full Name: histone deacetylase 10
• Calculated Molecular Weight: 669 aa, 71 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC125083
• Gene Symbol: HDAC10
• Gene ID (NCBI): 83933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.