CoraLite®555-conjugated HDAC1 Monoclonal antibody

HDAC1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4C4G7

Cat no : CL555-66085

Synonyms

DKFZp686H12203, GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Validation Data Gallery

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  IF Staining of A431 using CL555-66085

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 HDAC1 antibody (CL555-66085, Clone: 4C4G7 ) at dilution of 1:8000.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL555-66085 is HDAC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC1 fusion protein Ag19128
• Full Name: histone deacetylase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000301
• Gene Symbol: HDAC1
• Gene ID (NCBI): 3065
• RRID: AB_2919673
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .