HCN2 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84308-3-RR (same clone as 84308-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84308-3-RR (HCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84308-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00160-2

  Sandwich ELISA standard curve of MP00160-2, Human HCN2 Recombinant Matched Antibody Pair - PBS only. 84308-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34038. 84308-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP00160-2

  The mean HCN2 concentration was determined to be 9,765.2 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load and 23,767.3 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84308-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84308-3-RR against Human HCN2 were performed. The affinity constant is 0.795 nM.

HCN2 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84308-3-PBS

Clone No.241601E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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Product Information

84308-3-PBS targets HCN2 as part of a matched antibody pair:

MP00160-2: 84308-4-PBS capture and 84308-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HCN2 fusion protein Ag34038
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001194
• Gene Symbol: HCN2
• Gene ID (NCBI): 610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UL51
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)