HCN2 Polyclonal antibody

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  WB analysis using 30946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30946-1-AP (HCN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 30946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 30946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 30946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HCN2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30946-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30946-1-AP targets HCN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HCN2 fusion protein Ag34038
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 60 kDa, 90 kDa, 120 kDa
• GenBank Accession Number: NM_001194
• Gene Symbol: HCN2
• Gene ID (NCBI): 610
• RRID: AB_3086427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL51
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)

Publications

Species	Application	Title
mouse	WB	CNS Neurosci Ther; HCN channels in the lateral habenula regulate pain and comorbid depressive-like behaviors in mice; Authors - Xue-Zhong Cao