HCCS Polyclonal antibody

HCCS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15118-1-AP

Synonyms

Holocytochrome c-type synthase, Holocytochrome c type synthase, EC:4.4.1.17, Cytochrome c-type heme lyase, Cytochrome c type heme lyase

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Validation Data Gallery

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  WB analysis of human heart using 15118-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15118-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15118-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 15118-1-AP

  IP result of anti-HCCS (IP:15118-1-AP, 4ug; Detection:15118-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human breast cancer using 15118-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15118-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15118-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15118-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15118-1-AP (HCCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15118-1-AP targets HCCS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HCCS fusion protein Ag7189
• Full Name: holocytochrome c synthase (cytochrome c heme-lyase)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC001691
• Gene Symbol: HCCS
• Gene ID (NCBI): 3052
• RRID: AB_2114848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.

Publications

Species	Application	Title
human	WB	EMBO Mol Med; SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.; Authors - Alexandre Janer
human	WB	Chem Pharm Bull (Tokyo); The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo.; Authors - Bin Feng
human	WB	medRxiv; Biallelic variants in COX18 cause a mitochondrial disorder primarily manifesting as peripheral neuropathy; Authors - Camila Armirola-Ricaurte
human,mouse	WB	Nat Cell Biol; Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space; Authors - Elias Adriaenssens