HBG1/2-Specific Monoclonal antibody

HBG1/2-Specific Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B5

Cat no : 66168-1-Ig

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

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Validation Data Gallery

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1/2-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1/2-Specific antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66168-1-Ig targets HBG1/2-Specific in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hemoglobin, gamma A
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: NM_000559
• Gene Symbol: HBG1
• Gene ID (NCBI): 3047
• RRID: AB_2881564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.