HBG1/2-Specific Polyclonal antibody

HBG1/2-Specific Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 19993-1-AP

Synonyms

HBG1, HBGR, HBGA, Hb F Agamma, Gamma-1-globin

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Validation Data Gallery

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  WB analysis of recombinant proein using 19993-1-AP

  WB results of 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:2000.

• 

  WB analysis of human placenta using 19993-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 19993-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human HBG1/2-Specific (19993-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: recombinant proein, human placenta tissue
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19993-1-AP targets HBG1/2-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hemoglobin, gamma A
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: NM_000559
• Gene Symbol: HBG1
• Gene ID (NCBI): 3047
• RRID: AB_10949629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C