Hemoglobin Epsilon Polyclonal antibody

Hemoglobin Epsilon Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12361-1-AP

Synonyms

HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin

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Validation Data Gallery

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  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12361-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12361-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of K-562 using 12361-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Hemoglobin Epsilon (12361-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human placenta tissue, K-562 cells, mouse placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

12361-1-AP targets Hemoglobin Epsilon in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hemoglobin Epsilon fusion protein Ag3026
• Full Name: hemoglobin, epsilon 1
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 12-16 kDa
• GenBank Accession Number: BC015537
• Gene Symbol: HBE1
• Gene ID (NCBI): 3046
• RRID: AB_2114593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

Publications

Species	Application	Title
human	WB	Blood; NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression.; Authors - Linda Marie Starnes
human	WB	Cell Death Differ; Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.; Authors - L Vian
human	WB	Mol Cell Biol; A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.; Authors - Ma Yanni Y
human	WB	Biochim Biophys Acta Mol Cell Res; Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins.; Authors - Tetsuo Hirano
rat	WB	Proteome Sci; A comparison of E15.5 fetus and newborn rat serum proteomes.; Authors - Wei Lilong L
mouse	IHC	Blood; Requirement for antiapoptotic MCL-1 during early erythropoiesis; Authors - Meghan E Turnis

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