HBB Polyclonal antibody

HBB Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16216-1-AP

Synonyms

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin



Tested Applications

Positive WB detected inhuman heart tissue, human placenta tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16216-1-AP targets HBB in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HBB fusion protein Ag9216
Full Name hemoglobin, beta
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC007075
Gene Symbol HBB
Gene ID (NCBI) 3043
RRIDAB_10598329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

Protocols

Product Specific Protocols
WB protocol for HBB antibody 16216-1-APDownload protocol
IHC protocol for HBB antibody 16216-1-APDownload protocol
IF protocol for HBB antibody 16216-1-APDownload protocol
IP protocol for HBB antibody 16216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
mouseWB,IHC

Elife

ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription.

Authors - Rozenn Riou
humanWB

Stem Cells

Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.

Authors - Amy Leung

Sci Rep

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Authors - Inn-Inn Chen
mouseIHC

J Comp Neurol

Hemovasculogenic origin of blood vessels in the developing mouse brain.

Authors - Miguel A Gama Sosa
humanWB

Blood Cells Mol Dis

A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.

Authors - Tasha A Morrison