Hemoglobin Alpha-Specific Polyclonal antibody

Hemoglobin Alpha-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19990-1-AP

Synonyms

HBA1, Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin

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Validation Data Gallery

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  WB analysis of human heart using 19990-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19990-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 19990-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19990-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 19990-1-AP (Hemoglobin Alpha-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, human heart tissue, human spleen tissue
• Positive IHC detected in: human colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19990-1-AP targets Hemoglobin Alpha-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hemoglobin, alpha 1
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: NM_000558
• Gene Symbol: HBA1
• Gene ID (NCBI): 3039
• RRID: AB_11182500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.