Hemoglobin Alpha Recombinant antibody, PBS Only

Hemoglobin Alpha Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240049F12

Cat no : 83185-5-PBS

Synonyms

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

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Validation Data Gallery

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  WB analysis of human placenta using 83185-5-RR (same clone as 83185-5-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 83185-5-RR (same clone as 83185-5-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

• 

  FC experiment of TF-1 using 83185-5-RR (same clone as 83185-5-PBS)

  1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83185-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

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Product Information

83185-5-PBS targets Hemoglobin Alpha in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Full Name: hemoglobin, alpha 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-16 kDa, 27 kDa
• GenBank Accession Number: BC050661
• Gene Symbol: HBA1
• Gene ID (NCBI): 3039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.