HAX1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67883-2-Ig (same clone as 67883-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67883-2-Ig (same clone as 67883-2-PBS)

  WB result of HAX1 antibody (67883-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67883-2-Ig (same clone as 67883-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50775-2

  Cytometric bead array standard curve of MP50775-2, HAX1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67883-2-PBS. Detection antibody: 67883-5-PBS. Standard:Ag27244. Range: 0.391-50 ng/mL.

• Featured Product
• KD/KO Validated

HAX1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67883-2-PBS

Clone No.2A12F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HS1-associating protein X-1, HCLSBP1, HCLS1-associated protein X-1, HAX-1, HAX 1

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Product Information

67883-2-PBS targets HAX1 as part of a matched antibody pair:

MP50775-2: 67883-2-PBS capture and 67883-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HAX1 fusion protein Ag27244
• Full Name: HCLS1 associated protein X-1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014314
• Gene Symbol: HAX1
• Gene ID (NCBI): 10456
• RRID: AB_2918640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00165
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.