KIM-1/HAVCR1 Polyclonal antibody

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  WB analysis using 30948-1-AP

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 30948-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KIM-1/HAVCR1 antibody (30948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 30948-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KIM-1/HAVCR1 antibody (30948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIM-1/HAVCR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 30948-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

HAVCR1, KIM-1, TIM-1, TIM1, Kim1

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: rat kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

30948-1-AP targets KIM-1/HAVCR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: hepatitis A virus cellular receptor 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_173149.2
• Gene Symbol: Havcr1
• Gene ID (NCBI): 286934
• RRID: AB_3669790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O54947
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). KIM-1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

Publications

Species	Application	Title
rat	WB	Sci Total Environ; Exploring potential targets and mechanisms of renal tissue damage caused by N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone (6-PPDQ) through network toxicology and animal experiments: A case of chronic kidney disease; Authors - Jun Pei
mouse	IHC	Ren Fail; β-Hydroxybutyrate Protects Against Cisplatin-Induced Renal Damage via Regulating Ferroptosis; Authors - Ruixue Tian
rat	IHC	Ren Fail; Protective effect and mechanism of Xiaoyu Xiezhuo decoction on ischemia-reperfusion induced acute kidney injury based on gut-kidney crosstalk; Authors - Yue Ji
mouse	WB,IF	Int Immunopharmacol; BMAL1 alleviates sepsis-induced AKI by inhibiting ferroptosis; Authors - Songyuan Yang
mouse	WB,IF	Cell Signal; The SIRT6/BAP1/xCT signaling axis mediates ferroptosis in cisplatin-induced AKI; Authors - Songyuan Yang