HAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 11432-1-AP

Synonyms

KAT1, EC:2.3.1.48

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 11432-1-AP

  WB result of HAT1 antibody (11432-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

• 

  WB analysis using 11432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11432-1-AP (HAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11432-1-AP

  IP result of anti-HAT1 (IP:11432-1-AP, 4ug; Detection:11432-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 11432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11432-1-AP (HAT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse ovary using 11432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using HAT1 antibody (11432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11432-1-AP

  Immunofluorescent analysis of HepG2 cells, using HAT1 antibody 11432-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse ovary tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

11432-1-AP targets HAT1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAT1 fusion protein Ag1952
• Full Name: histone acetyltransferase 1
• Calculated Molecular Weight: 419 aa, 50 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018682
• Gene Symbol: HAT1
• Gene ID (NCBI): 8520
• RRID: AB_2116435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti
mouse	WB	Alcohol; Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.; Authors - Minjie Chen
human	IF	Biochem Biophys Res Commun; Identification and characterization of an endoplasmic reticulum localization motif.; Authors - Yong-Wei Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works very well to stain nuclei of developed oocytes in the mouse ovary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

HAT1 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:11432-1-AP)

FH

Leonardo (Verified Customer) (05-29-2019)

The antibody works well in a good range of sample (5-40 ug) in both cell lines I tested (MDA-MB-231 and HCC1806). I definitely recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: breast cancer cell lines

HAT1 Antibody Western Blot, validation (1:1000 dilution) in breast cancer cell lines (Cat no:11432-1-AP)