HAS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

HAS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15609-1-AP

Synonyms

HA synthase 3, HAS3, hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3

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Validation Data Gallery

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  WB analysis using 15609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15609-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15609-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, L02 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Product Information

15609-1-AP targets HAS3 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAS3 fusion protein Ag7999
• Full Name: hyaluronan synthase 3
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC021853
• Gene Symbol: HAS3
• Gene ID (NCBI): 3038
• RRID: AB_2232886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAS3(hyaluronan synthase 3) belongs to the NodC/HAS family, which are widely expressed in human tissues and appear to function in embryogenesis, wound healing and other processes associated with cellular proliferation and migration(PMID:14566823). HAS3 is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. It may play a role in the progression of colon cancer and, as such, may provide novel targets for diagnostic and therapeutic interventions(PMID:14566823). It has 2 isoforms produced by alternative splicing and the protein has a glycosylation site.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human,mouse	WB,IHC	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB	Int J Mol Sci; Elevation of Hyaluronan Synthase by Magnesium Supplementation Mediated through the Activation of GSK3 and CREB in Human Keratinocyte-Derived HaCaT Cells.; Authors - Kana Marunaka
human	WB,IHC,FC	Cancers (Basel); C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.; Authors - Romana Vidergar
		Invest Ophthalmol Vis Sci; PMID: 27092721; Authors - Anny M S Cheng
human	WB	Mol Med Rep; miR‑29a‑3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels.; Authors - Feihu Bai ; KD Validated