HARS Recombinant antibody, PBS Only (Detector)

HARS Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240495C3

Cat no : 83497-2-PBS

Synonyms

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP00486-1

  Sandwich ELISA standard curve of MP00486-1, Human HARS Recombinant Matched Antibody Pair - PBS only. 83497-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9333. 83497-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP00486-1

  MCF-7, Hela and HEK-293 cell lysates were measured. The human HARS concentration of detected samples was determined to be 579.62 ng/mL (based on a 4.3 mg/mL extract load) in MCF-7 cell lysate, 830.79 ng/mL (based on a 3.6 mg/mL extract load) in Hela cell lysate and 698.03 ng/mL (based on a 3.4 mg/mL extract load) in HEK-293 cell lysate.

• 

  Cytometric bead array standard curve of MP00486-1

  Cytometric bead array standard curve of MP00486-1, HARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83497-1-PBS. Detection antibody: 83497-2-PBS. Standard: Ag9333. Range: 0.78-100 ng/mL.

---

Product Information

83497-2-PBS targets HARS as part of a matched antibody pair:

MP00486-1: 83497-1-PBS capture and 83497-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HARS fusion protein Ag9333
• Full Name: histidyl-tRNA synthetase
• Calculated Molecular Weight: 509 aa, 57 kDa
• GenBank Accession Number: BC011807
• Gene Symbol: HARS
• Gene ID (NCBI): 3035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.