HARS Monoclonal antibody, PBS Only

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  WB analysis using 67662-1-Ig (same clone as 67662-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67662-1-Ig (same clone as 67662-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

HARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67662-1-PBS

Clone No.1B7G2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

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Product Information

67662-1-PBS targets HARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HARS fusion protein Ag9224
• Full Name: histidyl-tRNA synthetase
• Calculated Molecular Weight: 509 aa, 57 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC011807
• Gene Symbol: HARS
• Gene ID (NCBI): 3035
• RRID: AB_2882859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P12081
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.