HARBI1 Recombinant antibody

• 

  WB analysis using 84482-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84482-4-RR (HARBI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84482-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84482-4-RR against Human HARBI1 were performed. The affinity constant is 78.4 pM.

HARBI1 Recombinant Antibody for WB, ELISA

Cat No. 84482-4-RR

Clone No.241915C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative nuclease HARBI1, Harbinger transposase-derived nuclease, EC:3.1.-.-, C11orf77, 241915C10

---

Tested Applications

• Positive WB detected in: THP-1 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84482-4-RR targets HARBI1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HARBI1 fusion protein Ag34137
• Full Name: harbinger transposase derived 1
• Calculated Molecular Weight: 349 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC036925
• Gene Symbol: HARBI1
• Gene ID (NCBI): 283254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96MB7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HARBI1, also named as harbinger transposase derived 1, is predicted to enable metal ion binding activity and nuclease activity. HARBI1 is predicted to be involved in nucleic acid phosphodiester bond hydrolysis. [provided by Alliance of Genome Resources, Apr 2022] In plants, HARBI1 genes have developed new cellular functions to benefit the host, certain HARBI1 genes in rice and Arabidopsis thaliana ( A. thaliana) have been reported to play critical roles in regulating plant growth and development.(PMID:37950159)