HAPLN4 Polyclonal antibody

HAPLN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21228-1-AP

Synonyms

Brain link protein 2, BRAL2, HAPLN4, KIAA1926

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 21228-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21228-1-AP (HAPLN4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21228-1-AP is HAPLN4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAPLN4 fusion protein Ag15687
• Full Name: hyaluronan and proteoglycan link protein 4
• Calculated Molecular Weight: 402 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC142644
• Gene Symbol: HAPLN4
• Gene ID (NCBI): 404037
• RRID: AB_10695504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu