HAO2 Polyclonal antibody

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  WB analysis using 16563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16563-1-AP (HAO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16563-1-AP (HAO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16563-1-AP (HAO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16563-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HAO2 antibody (16563-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

HAO2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16563-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

(S)-2-hydroxy-acid oxidase, peroxisomal, 2-Hydroxyacid oxidase 2, Cell growth-inhibiting gene 16 protein, EC:1.1.3.15, GIG16

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, mouse kidney tissue, HepG2 cells, rat kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

16563-1-AP targets HAO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAO2 fusion protein Ag9838
• Full Name: hydroxyacid oxidase 2 (long chain)
• Calculated Molecular Weight: 351 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC020863
• Gene Symbol: HAO2
• Gene ID (NCBI): 51179
• RRID: AB_3669250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYQ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Arthritis Res Ther; Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning; Authors - Li Zhang