HAL Polyclonal antibody

HAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 25940-1-AP

Synonyms

HAL, HIS, Histidase, histidine ammonia lyase, HSTD

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Validation Data Gallery

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  WB analysis using 25940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25940-1-AP (HAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 25940-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25940-1-AP (HAL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skin using 25940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25940-1-AP (HAL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 25940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25940-1-AP (HAL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human skin tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 25940-1-AP is HAL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAL fusion protein Ag22985
• Full Name: histidine ammonia-lyase
• Calculated Molecular Weight: 657 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC096097
• Gene Symbol: HAL
• Gene ID (NCBI): 3034
• RRID: AB_2880303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa.

Publications

Species	Application	Title
human		Cell Death Dis; Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway; Authors - Guoqing Xie
	WB	Autophagy; The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple; Authors - Runmin Che
mouse	WB	FASEB J; Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment; Authors - Kenneth N Maclean

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (05-20-2022)

Very good antibody. Strong signal and clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse liver

HAL Antibody Western Blot validation (1:4000 dilution) in mouse liver (Cat no:25940-1-AP)