GLO2 Polyclonal antibody

GLO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17196-1-AP

Synonyms

HAGH, Glyoxalase II, GLXII, GLX2, Glx II

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Validation Data Gallery

• 

  WB analysis of mouse brain using 17196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17196-1-AP (GLO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17196-1-AP targets GLO2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLO2 fusion protein Ag9998
• Full Name: hydroxyacylglutathione hydrolase
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000840
• Gene Symbol: HAGH
• Gene ID (NCBI): 3029
• RRID: AB_2878361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLO2 (Glyoxalase 2) is one of the key enzymes in the glyoxalase system, which is mainly involved in cellular metabolic processes, especially the metabolic pathways related to glycolysis.

Publications

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human		Oncol Rep; Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cells; Authors - Yi-Jan Hsia