HADHA Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

HADHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B1

Cat no : 60250-1-PBS

Synonyms

GBP, ECHA, EC:2.3.1.-, 78 kDa gastrin-binding protein, 78 kDa gastrin binding protein

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Validation Data Gallery

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  WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

  WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60250-1-Ig (same clone as 60250-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50412-1

  Cytometric bead array standard curve of MP50412-1, HADHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60250-1-PBS. Detection antibody: 60250-2-PBS. Standard:Ag1211. Range: 1.563-100 ng/mL.

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Product Information

60250-1-PBS targets HADHA as part of a matched antibody pair:

MP50412-1: 60250-1-PBS capture and 60250-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HADHA fusion protein Ag1211
• Full Name: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC009235
• Gene Symbol: HADHA
• Gene ID (NCBI): 3030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).