HACE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HACE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24104-1-AP

Synonyms

HACE1, KIAA1320

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Validation Data Gallery

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  WB analysis of HEK-293 using 24104-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24104-1-AP (HACE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24104-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24104-1-AP (HACE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24104-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24104-1-AP (HACE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 24104-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24104-1-AP (HACE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 24104-1-AP

  Immunofluorescent analysis of HeLa cells using 24104-1-AP (HACE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 24104-1-AP is HACE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HACE1 fusion protein Ag21130
• Full Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 909 aa, 102 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC034982
• Gene Symbol: HACE1
• Gene ID (NCBI): 57531
• RRID: AB_2879432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HACE1(E3 ubiquitin-protein ligase HACE1) also named as KIAA1320, has a tumor-suppressor function dependent on its E3 ligase activity and controlling cell cycle progression during cell stress through degradation of cyclin D1. It belongs to the HECT family of E3 ubiqutin protein ligase. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 102 kDa, 36 kDa, 64 kDa and 78 kDa.

Publications

Species	Application	Title
mouse	WB	Reprod Biol Endocrinol; NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes; Authors - Shiwei Wang ; KD Validated
human	WB	Acta Biochim Biophys Sin (Shanghai); Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression; Authors - Kenan Huang
human	IHC	Cancer Med; Overexpression of HACE1 in gastric cancer inhibits tumor aggressiveness by impeding cell proliferation and migration.; Authors - Ying-Ling Chen ; KO Validated
human	IHC	Transl Cancer Res; Unveiling the role of HACE1 in cervical cancer: implications for human papillomavirus infection and prognosis; Authors - Siyang Xiang