HABP2 Monoclonal antibody

HABP2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A7C2

Cat no : 67867-1-Ig

Synonyms

FSAP, Factor VII-activating protease, Factor VII activating protease, Factor seven-activating protease, EC:3.4.21.-

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Validation Data Gallery

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  WB analysis of human plasma using 67867-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 67867-1-Ig (HABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67867-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HABP2 antibody (67867-1-Ig, Clone: 1A7C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 67867-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug HABP2 Monoclonal antibody (67867-1-Ig, Clone:1A7C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67867-1-Ig targets HABP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HABP2 fusion protein Ag3981
• Full Name: hyaluronan binding protein 2
• Calculated Molecular Weight: 560 aa, 63 kDa
• Observed Molecular Weight: 60,63 kDa
• GenBank Accession Number: BC031412
• Gene Symbol: HABP2
• Gene ID (NCBI): 3026
• RRID: AB_2918625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.