HAAO Polyclonal antibody

• Featured Product
• KD/KO Validated

HAAO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12791-1-AP

Synonyms

EC:1.13.11.6, 3-hydroxyanthranilic acid dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO

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Validation Data Gallery

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  WB analysis of human liver using 12791-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12791-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 12791-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12791-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 12791-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12791-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12791-1-AP (HAAO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

12791-1-AP targets HAAO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAAO fusion protein Ag3511
• Full Name: 3-hydroxyanthranilate 3,4-dioxygenase
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC029510
• Gene Symbol: HAAO
• Gene ID (NCBI): 23498
• RRID: AB_2115432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth; Authors - Dong Liu ; KO Validated
rat	IHC	BMC Genomics; Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats; Authors - Wang Zhu
human	WB	Nat Commun; Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma; Authors - Miyuki Nomura
mouse	WB	Environ Pollut; Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis; Authors - Zheng Yu