H6PD Recombinant antibody

H6PD Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C14

Cat no : 82819-2-RR

Synonyms

6PGL, DKFZp686A01246, G6PDH, GDH, H6PD

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Validation Data Gallery

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  WB analysis using 82819-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82819-2-RR (H6PD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82819-2-RR targets H6PD in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: H6PD fusion protein Ag7109
• Full Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89-95 kDa
• GenBank Accession Number: BC081559
• Gene Symbol: H6PD
• Gene ID (NCBI): 9563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD).