Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4C7G2

Cat no : 66863-1-Ig

Synonyms

H3 S10, H3 Ser10, H3/A, H3FA, H3S10, HIST1H3A, histone, histone cluster 1, H3a, Histone H3.1, Histone H3/a, PHH3, Phospho-Histone H3 Ser10



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, Jurkat cells, human renal cell carcinoma tissue, mouse kidney tissue, rat kidney tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, HeLa cells, A549 cells, MCF-7 cells, SKOV-3 cells, mouse testis tissue, C2C12 cells
Positive FC detected inHepG2 cells, nocodazole treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66863-1-Ig targets Phospho-Histone H3 (Ser10) in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Reactivity Human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberNM_003529
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2882201
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-IgDownload protocol
IHC protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-IgDownload protocol
IF protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncogene

Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer.

Authors - Jun You
humanWB

Biochem Pharmacol

A novel aromatic amide derivative SY-65 co-targeted tubulin and histone deacetylase 1 with potent anticancer activity in vitro and in vivo.

Authors - Yin-Ru Li
humanIF

Front Mol Biosci

High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy.

Authors - Steven J Edwards
mouseIF

Sci Rep

NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.

Authors - Sylvia Lai
chickenIHC

Dev Dyn

The dynamic spatial and temporal relationships between the phalanx-forming region and the interdigits determine digit identity in the chick limb autopod.

Authors - Takayuki Suzuki
mouseWB

Front Immunol

Oocytes could rearrange immunoglobulin production to survive over adverse environmental stimuli

Authors - Yang Wang