Histone H2A.z Polyclonal antibody

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  WB analysis of human brain using 16441-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16441-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16441-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 16441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 16441-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Histone H2A.z Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16441-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

H2AFZ, Histone H2A, H2AZ1, H2AZ, H2A/z

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Tested Applications

• Positive WB detected in: human brain tissue, mouse kidney tissue
• Positive IHC detected in: human skin cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

16441-1-AP targets Histone H2A.z in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, fish, oratosquilla oratoria
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H2A.z fusion protein Ag9762
• Full Name: H2A histone family, member Z
• Calculated Molecular Weight: 128 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC018002
• Gene Symbol: Histone H2A.z
• Gene ID (NCBI): 3015
• RRID: AB_2115113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P0C0S5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
fish	IF	Sci Adv; The lungfish cocoon is a living tissue with antimicrobial functions.; Authors - Ryan Darby Heimroth
mouse	WB	J Biol Chem; The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.; Authors - Haixin Zhao ; KD Validated
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
mouse	IF,WB	Biol Open; Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway.; Authors - BreAnna Cameron
Oratosquilla oratoria	IF	J Morphol; Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).; Authors - Tingrong Chen
human	WB	Front Oncol; DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway; Authors - Zhitao Han