Histone H2A.X Polyclonal antibody

Histone H2A.X Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

122

Reviews (2)

Cat no : 10856-1-AP

Synonyms

H2AFX, Histone H2AX, Histone H2A, H2AX, H2A.X

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Validation Data Gallery

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  WB analysis using 10856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10856-1-AP (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H2A.X antibody (10856-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of U-251 using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using 10856-1-AP (Histone H2A.X antibody), at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells, HEK-293 ells, mouse heart, mouse kidney, rat kidney
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 90 publications below
• IHC: See 11 publications below
• IF: See 34 publications below
• ChIP: See 1 publications below

Product Information

10856-1-AP targets Histone H2A.X in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H2A.X fusion protein Ag1305
• Full Name: H2A histone family, member X
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC013416
• Gene Symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• RRID: AB_2114985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

Publications

Species	Application	Title
human	WB,IHC,IF	ACS Nano; Polyoxometalate-Based Radiosensitization Platform for Treating Hypoxic Tumors by Attenuating Radioresistance and Enhancing Radiation Response.; Authors - Yuan Yong
mouse	IF	ACS Nano; A Heterojunction Structured WO2.9-WSe2 Nanoradiosensitizer Increases Local Tumor Ablation and Checkpoint Blockade Immunotherapy upon Low Radiation Dose.; Authors - Xinghua Dong
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
mouse	IF	ACS Appl Mater Interfaces; Extremely Effective Chemoradiotherapy by Inducing Immunogenic Cell Death and Radio-Triggered Drug Release under Hypoxia Alleviation; Authors - Chunqi Zhu
human	WB	Cancer Res; ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth.; Authors - Yong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andreas (Verified Customer) (03-23-2022)

Antibody gives very good signal, hence the dilution factor. One important note is that I include 1:2000 dilution of benzonase in my lysis buffer to recover most of the chromatin-bound material, which big part of it is otherwise lost during the clarification step.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293FT

FH

kes (Verified Customer) (01-17-2022)

used for WB (1:1000) in milk+TBST buffer and got good results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LIver cell lines

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