Histone H1.0 Polyclonal antibody

Histone H1.0 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 17510-1-AP

Synonyms

H1F0, Histone H1, H1FV, H1-0, H10

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Validation Data Gallery

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  WB analysis of HeLa using 17510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17510-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17510-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17510-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17510-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17510-1-AP

  IP result of anti-Histone H1.0 (IP:17510-1-AP, 4ug; Detection:17510-1-AP 1:500) with A431 cells lysate 2400ug.

• 

  IHC staining of human colon using 17510-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17510-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 17510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 17510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, A431 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: skin cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

17510-1-AP targets Histone H1.0 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H1.0 fusion protein Ag9982
• Full Name: H1 histone family, member 0
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000145
• Gene Symbol: Histone H1.0
• Gene ID (NCBI): 3005
• RRID: AB_10695628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
human	WB	Theranostics; PMID: 29290796; Authors - Jin Zeng
human	WB	Nucleic Acids Res; Pin1 promotes GR transactivation by enhancing recruitment to target genes.; Authors - Poolman Toryn M TM
human	WB,IF	Oncogene; Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.; Authors - Xianchong Zheng
human	WB	PLoS Pathog; Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein.; Authors - Linyuan Feng
human	WB	Front Cell Dev Biol; GATA4 Regulates Inflammation-Driven Pancreatic Ductal Adenocarcinoma Progression.; Authors - Weiliang Jiang