Phospho-GSK3B (Ser389) Polyclonal antibody

Phospho-GSK3B (Ser389) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

38

Cat no : 14850-1-AP

Synonyms

7330414F15Rik, 8430431H08Rik, C86142, Gsk3b, Ser389

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Validation Data Gallery

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  WB analysis using 14850-1-AP

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14850-1-AP (Phospho-GSK3B (Ser389) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

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Tested Applications

• Positive WB detected in: Calyculin A treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 38 publications below

Product Information

14850-1-AP targets Phospho-GSK3B (Ser389) in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC060743
• Gene Symbol: Gsk3b
• Gene ID (NCBI): 56637
• RRID: AB_2878085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase 3s are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3α and GSK3β, encoded by separate genes, which are overall 85% homologous to each other, with 95% identity in the kinase domains. To date, phosphorylation at Ser9 by Akt is the best-characterized mechanism for the inhibition of GSK3β activity. GSK3β is phosphorylated at S389 in vivo by p38 MAPK and that this alternative regulatory mechanism of GSK3β is tissue-specific(PMID:18451303).

Publications

Species	Application	Title
mouse	WB	Redox Biol; Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different Aβ concentrations.; Authors - Kim Cuong Cap
human	WB	Cancer Res; p38 Stabilizes Snail by Suppressing DYRK2-Mediated Phosphorylation that is Required for GSK3β-βTrCP-Induced Snail Degradation.; Authors - Ki-Jun Ryu
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	Int J Mol Sci; GSK3β Serine 389 Phosphorylation Modulates Cardiomyocyte Hypertrophy and Ischemic Injury.; Authors - Laura Vainio
human	WB	Front Oncol; FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.; Authors - Yi-Wen Zheng
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong