Granzyme B Recombinant antibody

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  WB analysis of NK-92 using 83763-5-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 83763-5-RR (GZMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83763-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83763-5-RR against Human Granzyme B were performed. The affinity constant is 0.281 nM.

Granzyme B Recombinant Antibody for WB, ELISA

Cat No. 83763-5-RR

Clone No.240761C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

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Tested Applications

• Positive WB detected in: NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83763-5-RR targets Granzyme B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• RRID: AB_3671358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10144
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.